ABSTRACT: Effect of gamma rays on Soybean (Glycine max (L.) Mrr.) var. Co-1plants of three generation viz., M1, M2 and M3, The mutagens like Gamma rays were treatments for various doses (10,20,30,40,50,60,70,80,90 and 100KR) separately. The morphology and yield parameters were carried out M1, M2 and M3 generations. The effect of mutagens on different growth parameters, yields and yield components and quality (protein and oil) were studied. The seeds were grown in the field and observation separate concentration. The following characters were recorded such as days to first followers, plant height, number of leaves per plant, number of branches per plant, number of cluster per plant, number of pods per plant, number of seeds per plant, seed yield per plant, protein content and oil content. The yield parameters like plant height, number of cluster per plant, number of seeds per plant and seed yield per plant were recorded the moderated and high mean value in the 50 KR of treated population of all generation with compared to control plants.